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第一作者:Gregory Reeves
第一单位:剑桥大学
第一通讯:Julian M. Hibberd
Abstract
背景回顾:Grafting is possible in both animals and plants. Although in animals the process requires surgery and is often associated with rejection of non-self, in plants grafting is widespread, and has been used since antiquity for crop improvement. 提出问题:However, in the monocotyledons, which represent the second largest group of terrestrial plants and include many staple crops, the absence of vascular cambium is thought to preclude grafting. 主要发现:Here we show that the embryonic hypocotyl allows intra- and inter-specific grafting in all three monocotyledon groups: the commelinids, lilioids and alismatids. 结果1-功能性嫁接体:We show functional graft unions through histology, application of exogenous fluorescent dyes, complementation assays for movement of endogenous hormones, and growth of plants to maturity. 结果2-嫁接分子响应:Expression profiling identifies genes that unify the molecular response associated with grafting in monocotyledons and dicotyledons, but also gene families that have not previously been associated with tissue union. 结果3-嫁接价值:Fusion of susceptible wheat scions to oat rootstocks confers resistance to the soil-borne pathogen Gaeumannomyces graminis. 结论:Collectively, these data overturn the consensus that monocotyledons cannot form graft unions, and identify the hypocotyl (mesocotyl in grasses) as a meristematic tissue that allows this process. We conclude that graft compatibility is a shared ability among seed-bearing plants. 摘 要
嫁接在动物和植物中都是可能的。尽管在动物中,嫁接需要进行手术,并且时有发生排异反应;但是在植物中嫁接非常普遍,自古以来就被用于作物的改良。但是,陆地植物中第二大类群、包含很多主粮作物的单子叶植物,由于缺少维管形成层而被认为不能进行嫁接。本文中,作者发现在单子叶三大类群鸭跖草分支、百合分支和泽泻分支物种中,通过胚性下胚轴均能够进行种内或种间的嫁接。作者通过组织学、外源荧光染料、内源激素移动的互补分析,以及让嫁接植物发育至成熟等试验,进一步证明了单子叶植物嫁接体的功能性。表达谱分析鉴定了单子叶和双子叶植物对于嫁接统一的分子响应,但同时也鉴定到了一些之前未曾发现的与嫁接体相关的基因家族。通过将易感的小麦接穗与具抗性的燕麦砧木嫁接,可获得对土传病原菌禾顶囊壳的抗性。综上,本文的研究结果打破了先前认为单子叶植物不能进行嫁接的共识,发现下胚轴作为分生组织能够使得单子叶植物进行嫁接。因此,作者总结道,嫁接亲和是所有种子植物的共有能力。
通讯作者
** Julian M. Hibberd **
个人简介: 英国班戈大学,博士。 研究方向:C4植物的光合作用。
doi: https://doi.org/10.1038/s41586-021-04247-y
Journal: Nature
Published date: December 22, 2021
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