Highlight: This is an easy-understanding paper, describing a detailed process for analyzing allele-specific expression with RNA-Seq data. It is very useful and it is possible for us to follow its steps to achieve the preliminary results. Outline: I. Expectation: a large fraction of the loci (SNPs) ...
Outline: I. Data 1) Data source: the authors sequenced the mRNA fraction of the transcriptome of lymphoblastoid cell lines from 60 CEU individuals using 37-bp paired-end Illumina sequencing. Each individual yielded 16.9+-5.9 million reads that mapped to the NCBI36 assembly of the human genome using ...
Summary in one sentence: It is a review generally reviewing the methods for genome-wide allele-specific analysis and the mechanisms causing the allelic biases. Outlines: 1. eQTL is what researchers are focusing on to find out human genetic variation that affects gene expression. However, direct ass ...