▲ Vol 26 Issue 02 |  February , 2024

Single-cell multi-omics profiling of human preimplantation embryos identifies cytoskeletal defects during embryonic arrest

Teng Wang, Junhua Peng, Jiaqi Fan, Ni Tang, Rui Hua, Xueliang Zhou, Zhihao Wang, Longfei Wang, Yanling Bai, Xiaowan Quan, Zimeng Wang, Li Zhang, Chen Luo, Weiqing Zhang, Xiangjin Kang, Jianqiao Liu, Lei Li & Lin Li

Human in vitro fertilized embryos exhibit low developmental capabilities, and the mechanisms that underlie embryonic arrest remain unclear. Here using a single-cell multi-omics sequencing approach, we simultaneously analysed alterations in the transcriptome, chromatin accessibility and the DNA methylome in human embryonic arrest due to unexplained reasons. Arrested embryos displayed transcriptome disorders, including a distorted microtubule cytoskeleton, increased genomic instability and impaired glycolysis, which were coordinated with multiple epigenetic reprogramming defects. We identified Aurora A kinase (AURKA) repression as a cause of embryonic arrest. Mechanistically, arrested embryos induced through AURKA inhibition resembled the reprogramming abnormalities of natural embryonic arrest in terms of the transcriptome, the DNA methylome, chromatin accessibility and H3K4me3 modifications. Mitosis-independent sequential activation of the zygotic genome in arrested embryos showed that YY1 contributed to human major zygotic genome activation. Collectively, our study decodes the reprogramming abnormalities and mechanisms of human embryonic arrest and the key regulators of zygotic genome activation.

‍https://www.nature.com/articles/s41556-023-01328-0‍

▲ Vol 40 Issue 02 |  February , 2024

Three-dimensional morphogenesis of epithelial tubes

Pengyu Yu & Bo Li

Epithelial tubes serve as fundamental structures within diverse organs. Morphogenesis of epithelial tubes involves cell deformations, biochemical signaling, and the cross-talking between cells and the extracellular matrix. However, it remains incompletely understood how the interplay between mechanics and biochemical signaling modulates the morphologies of epithelial tubes. In this work, we develop a three-dimensional (3D) vertex model incorporating biochemical signaling pathways to investigate epithelial tube morphogenesis. We reveal that the mechanical properties of both cells and the apical extracellular matrix can regulate the size of the tube. The chemomechanical deformation of cells can activate supercellular spot and stripe actomyosin patterns, which, consequently, induce the wave- or ring-shaped tube configurations, depending on diffusion of chemical species. Our study highlights the significant role of mechano-chemical interplay in morphodynamics of tissues and also provides a 3D framework to decode complex pattern formation in biological structures.

https://link.springer.com/article/10.1007/s10409-023-23297-x

▲ Vol 57 Issue 03 | 13 February , 2024

Large-Scale Synthesis of Reactive Janus Inorganic/Polymer Colloidal Dimer

Yajing Yang, Fenglin Li, Dayin Sun, Yilan Ye, and Zhenzhong Yang

A new method is proposed to large-scale synthesize reactive Janus inorganic/polymer colloidal dimers by electrostatics-mediated intramolecular crosslinking of the tethered diblock copolymer single-chain of PVSt-b-PmSt at an example inorganic NP of Fe3O4@SiO2. Tethering the single-chain is achieved by terminating the anionic living polymer onto the chloromethylphenyl- group capped NP. While the electrostatic interaction is introduced by complexation of the mercaptoacetic acid (MA) clicked PVSt block with 1-methylimidazole (MI), the intramolecular crosslinking is achieved with 1,6-hexanediisothiocyanate (HDI). The example dimer of cPVSt-b-PmSt-Fe3O4@SiO2 could be synthesized in a highly concentrated dispersion up to 100 mg/mL. The amphiphilic Janus colloidal dimer is effective to manipulate the stabilized emulsion droplets with a magnet. The parent colloidal dimer is highly reactive with residual chloromethylphenyl-, cyanate, and carboxylic acid groups at different sites to assist deriving a family of functional dimers by simple modifications.

https://pubs.acs.org/doi/10.1021/acs.macromol.3c02334

▲ Vol 04 Issue 02 | 16 February , 2024

Photo-driven Surfactant-Free Gold Nanostars for Rapid Bacterial Detection in Food Safety Using Surface-Enhanced Raman Spectroscopy

Zhengyi Chen, Laishui Yu, Zhi Zhang, Linjing Su, and Yuhao Xiong

Traditional foodborne bacterial detection methods have limitations, driving the need for advanced techniques like surface-enhanced Raman spectroscopy (SERS), known for its high sensitivity, specificity, and nondestructive analysis in food safety. However, SERS faces challenges regarding substrate sensitivity and complex sample preparation. This study presents an innovative photo-driven method for synthesizing surfactant-free anisotropic gold nanoparticles for SERS applications. These gold nanostars, with unique morphology and superior SERS performance, are used for sensitive Escherichia coli (E. coli) detection in complex food samples. Gold nanostar synthesis, controlled by the pH, reaction time, and HAuCl4 concentration, results in enhanced SERS capabilities. For in situ indole separation, a metabolic product of E. coli and the detection target, we introduce a miniaturized array gas membrane separation device. The SERS-based E. coli detection method exhibits remarkable sensitivity, with a 5 cfu/mL limit of detection and reliability within a 100–800 cfu/mL concentration range.

https://pubs.acs.org/doi/10.1021/acsfoodscitech.3c00470

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静远嘲风（MY Scimage） 成立于2007年，嘲风取自中国传统文化中龙生九子，子子不同的传说，嘲风为守护屋脊之瑞兽，喜登高望远；静远取自成语“宁静致远”，登高莫忘初心，远观而不可务远。

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