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上传于 2012-3-13 01:29 (54 KB)

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IP: 46.7.101.*   JANEWANGJK55 发表了评论   2012-3-13 01:41
Basic Conception

1, Relaxosome:
Relaxosome is the complex of proteins that facilitates plasmids during bacterial conjugation. The proteins are encoded by the tra gene in the origin of transfer, oriT, region. The most important of these proteins is relaxase, which is responsible for beginning the conjugation process by cutting at the nic site via transesterification. This nicking results in a DNA-Protein complex with the relaxosome bound to a single strand of the plasmid DNA and an exposed 3' hydroxyl group. Relaxase also unwinds the plasmid being conjugated with its helicase properties. The relaxosome interacts with integration host factors within the oriT.
                                                                  ---------From Wikipedia, the free encyclopedia
IP: 46.7.101.*   JANEWANGJK55 发表了评论   2012-3-13 01:52
Incompatibility typing proved to be useful since specific plasmid types have been linked with antimicrobial resistance and virulence (Couturier et al., 1988; Gotz et al., 1996; Carattoli et al., 2005)
IP: 193.1.165.*   JANEWANGJK55 发表了评论   2012-3-18 20:46
http://www.ucd.ie/cfs/people/
IP: 193.1.165.*   JANEWANGJK55 发表了评论   2012-3-19 19:50
http://www.ecoliblog.com/
IP: 193.1.165.*   JANEWANGJK55 发表了评论   2012-3-27 23:24
Double-disk synergy test ?
IP: 193.1.164.*   JANEWANGJK55 发表了评论   2012-3-28 20:15
http://www.breakingnews.ie/ireland/ucd-team-tackles-antibiotic-resistance-545243.html
IP: 193.1.165.*   JANEWANGJK55 发表了评论   2012-3-29 19:58
http://www.encyclopedia.com/doc/1G2-3409800162.html
IP: 193.1.165.*   JANEWANGJK55 发表了评论   2012-4-3 00:09
2012-04-02
a, take all the strains and stuffs from M
b, write Chapters 11 & 12 which will go directly to your dissertation
c, heat map for resistance to R (go back to S, when you get the results)
d, try to think about the paper
e, Biomerieux
IP: 46.7.101.*   JANEWANGJK55 发表了评论   2012-4-4 08:44
2012-04-04 Morning
a, calculate the frequency
b, heat map making
c, Biomerieux project checking
IP: 193.1.164.*   JANEWANGJK55 发表了评论   2012-4-11 18:11
Before 2012-04-13
a, chapters
b, data processing
c, PFGE
IP: 193.1.164.*   JANEWANGJK55 发表了评论   2012-4-13 22:29
in April>
a, chapter
b, review
c, STA report
d, project update presentation
IP: 193.1.164.*   JANEWANGJK55 发表了评论   2012-4-14 00:07
Just finished to talk to boss,
a, go on for the chapter (useful for your STA report)
b, add M's transconjugants into your heat-map, compare the different sources & why they could make that on Monday
c, think about how to put your samples into Bio.... ask E about that and learn
IP: 193.1.164.*   JANEWANGJK55 发表了评论   2012-4-16 22:33
Just finished to talk to M,
a, May need to restreak her trans from -20oC
b, How to find the one to sequence:
   Compare the incompatibility type and resistance profiles with the ones in PUBMAD
   Find the GAP which may interesting
c, you may chage 150kb if you find the more accurate size
IP: 193.1.164.*   JANEWANGJK55 发表了评论   2012-4-16 22:36
d, E-test may should do triple (try to find if the resistance doulbed or not)
e, Don't waste M from the grand, however, don't waste your Experiments' Results either!
IP: 193.1.164.*   JANEWANGJK55 发表了评论   2012-4-16 22:55
f, the manual of Bio.... is very useful
IP: 193.1.165.*   JANEWANGJK55 发表了评论   2012-5-1 00:44
in May&June>
For Writing
a, CHAPTERS
b, STA REPORT
c, PROJECT PAPER
IP: 193.1.165.*   JANEWANGJK55 发表了评论   2012-5-1 17:38
写论文就是写小说。武侠风格尤佳。首先营造一个紧张的氛围,烘托出一个必须解决的严重问题。接着绿叶登场,挨个败下阵来。然后主人公出现,在精巧的情节布局之下,他的特长刚好得到最大限度地发挥,他的缺陷刚好都不重要,甚至还能变废为宝。于是主人公拯救了学术界,并在剧终谦虚地表示,由于时间有限自己的独门武功还有6层没有修炼完。
IP: 193.1.165.*   JANEWANGJK55 发表了评论   2012-5-10 23:01
PRESENTATION IN 10/05/2012   SPEAK SLOWLY AND CORRECTLY  
a, Report to Roger
b, Chapter 242
c, STA report

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