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IP: 5.179.69.*   JANEWANGJK55 发表了评论   2014-8-14 22:25
maternity  [ma·ter·ni·ty || mə'tɜrnətɪ /-'tɜːn-]
n.  母性, 妇产科医院, 母道
adj.  产妇的
IP: 5.179.69.*   JANEWANGJK55 发表了评论   2014-8-14 22:29
chino  
n.  斜纹棉布; 斜纹棉布裤
IP: 5.179.69.*   JANEWANGJK55 发表了评论   2014-8-14 22:54
exclusive  [ex·clu·sive || ɪk'skluːsɪv]
adj.  独占的, 排外的, 唯一的
IP: 137.43.16.*   JANEWANGJK55 发表了评论   2014-9-5 00:16
aadA1  ----- streptomycin and spectinomycin
IP: 137.43.16.*   JANEWANGJK55 发表了评论   2014-9-5 00:16
pCL1920    4.6 kb
pCL1921    4.6 kb
IP: 137.43.16.*   JANEWANGJK55 发表了评论   2014-9-5 00:17
distal ---- downstream
proximal -----  upsream
IP: 137.43.16.*   JANEWANGJK55 发表了评论   2014-9-5 00:19
ushsevenaec95
IP: 5.179.69.*   JANEWANGJK55 发表了评论   2014-11-24 18:49
abcdefghijklmnopqrstuvwxyzzyxwvutsrqponmlkjihgfedcba
IP: 5.179.69.*   JANEWANGJK55 发表了评论   2014-11-24 18:49
ABCDEFG   HIJKLMN   OPQRST   UVWXYZ
IP: 5.179.69.*   JANEWANGJK55 发表了评论   2014-11-27 03:46
刚刚 任大哥说他明年2015年三月份,写完论文初稿! 以此为据!
IP: 5.179.69.*   JANEWANGJK55 发表了评论   2014-11-27 03:47
    坐等任大哥的PHD 毕业!  
IP: 5.179.69.*   JANEWANGJK55 发表了评论   2014-11-27 03:57
列字据:
王小娟和任小渊,2014年12月21日,22日,23日均要去办公室工作。
2014年12月24日,25日,26日圣诞节休息三天。
2014年12月27日,28日,29日, 30日,31日均要去办公室工作。
2015年1月1日,2日元旦休息两天。
2105年1月3日,4日均去办公室工作。
以此为据! 望王小娟和任小渊监督好彼此!
PS. 在假期期间,鉴于王小娟需要完成的任务量比较小,故王小娟在极度想休闲的时候,可以特另批准。但,王小娟应做好对任小渊的监督管理工作! ^_^
IP: 137.43.207.*   JANEWANGJK55 发表了评论   2014-11-28 18:45
The following abbreviations are commonly used to describe the length of a D/RNA molecule:

bp = base pair(s)— one bp corresponds to approximately 3.4 Å (340 pm) of length along the strand, and to roughly 618 or 643 daltons for DNA and RNA respectively.
kb (= kbp) = kilo base pairs = 1,000 bp
Mb = mega base pairs = 1,000,000 bp
Gb = giga base pairs = 1,000,000,000 bp.
IP: 137.43.183.*   JANEWANGJK55 发表了评论   2014-12-12 04:13
http://integrall.bio.ua.pt/?glossary
IP: 5.179.69.*   JANEWANGJK55 发表了评论   2015-1-11 23:22
http://www.amazon.co.uk/Madame-Curie-Complex-published-Feminist/dp/B00E327OD2/ref=sr_1_2?s=books&ie=UTF8&qid=1420989603&sr=1-2&keywords=The+Madame+Curie+Complex%3A+The+Hidden+History+of+Women+in+Science
Book that I want to buy  :)
IP: 137.43.46.*   JANEWANGJK55 发表了评论   2015-6-12 16:36
http://mlst.warwick.ac.uk/mlst/dbs/Ecoli/documents/primersColi_html
MLST Databases at UoW
Protocols used for MLST of Escherichia coli


Genes

The E. coli MLST scheme uses internal fragments of the following seven house-keeping genes:

adk (adenylate kinase)
fumC (fumarate hydratase)
gyrB (DNA gyrase)
icd (isocitrate/isopropylmalate dehydrogenase)
mdh (malate dehydrogenase)
purA (adenylosuccinate dehydrogenase)
recA (ATP/GTP binding motif)


PCR Amplification

Please note: These include new primer sequences (added 26 July 2004; labelling corrected on 5 March, 2007) whose labels indicate the genomic direction rather than reading frame.
The primer pairs for the PCR amplification of internal fragments of these genes can be chosen from:


Gene:        Product length:        Annealing Temperature:

adkF 5'-ATTCTGCTTGGCGCTCCGGG-3'
adkR 5'-CCGTCAACTTTCGCGTATTT-3'
adkF1 5'-TCATCATCTGCACTTTCCGC-3'
adkR1 5'-CCAGATCAGCGCGAACTTCA-3'

583 bp        54° C
fumCR1 5'-TCCCGGCAGATAAGCTGTGG-3'
fumCF 5'-TCACAGGTCGCCAGCGCTTC-3'
fumCR 5'-GTACGCAGCGAAAAAGATTC-3'

806 bp        54° C
gyrBF 5'-TCGGCGACACGGATGACGGC-3'
gyrBR1 5'-GTCCATGTAGGCGTTCAGGG-3'
gyrBR 5'-ATCAGGCCTTCACGCGCATC-3'

911 bp        60° C
icdF 5'-ATGGAAAGTAAAGTAGTTGTTCCGGCACA-3'
icdR 5'-GGACGCAGCAGGATCTGTT-3'

878 bp        54° C
mdhF 5'-ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG-3'
mdhR 5'-TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT-3'
mdhF1 5'-AGCGCGTTCTGTTCAAATGC-3'
mdhR1 5'-CAGGTTCAGAACTCTCTCTGT-3'

932 bp        60° C
purAF1 5'-TCGGTAACGGTGTTGTGCTG-3'
purAF 5'-CGCGCTGATGAAAGAGATGA-3'
purAR 5'-CATACGGTAAGCCACGCAGA-3'

816 bp        54° C
recAR1 5'-AGCGTGAAGGTAAAACCTGTG-3'
recAF 5'-CGCATTCGCTTTACCCTGACC-3'
recAF1 5'-ACCTTTGTAGCTGTACCACG-3'
recAR 5'-TCGTCGAAATCTACGGACCGGA-3'        780 bp        58° C

Conditions:

PCR: 2 min at 95°, 30 cycles of 1 min at 95°, 1 min at annealing temp, 2 min at 72° followed by 5 min at 72°. The PCR reaction contains 50 ng of chromosomal DNA, 20 pmol of each primer, 200 umol (10 ul of a 2 mM solution) of the dNPTs, 10 ul of 10x PCR buffer, 5 units of Taq polymerase and water to 100 ul.

Sequencing

We use the amplification primer pairs for the sequencing step.

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