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- JANEWANGJK55
- E. coli CFSAN004177 plasmid PCFSAN004177G_03 (CP012494) 96,228bp.
E. coli strain CFSAN004176 plasmid pCFSAN004176P_03 (CP012491.1) Length: 95721,
Escherichia coli str. Sanji plasmid pSJ_98, Sequence ID: CP011063.1Length: 98436.
Escherichia coli genome assembly FHI87, scaffold scaffold-11_contig-13.0_1_90875, Sequence ID: LM996988.1Length: 90875
Salmonella phage SJ46, complete genome, Sequence ID: KU760857.1Length: 103445
Escherichia coli strain ST2747, complete genome, Sequence ID: CP007392.1Length: 5054509
Escherichia coli strain ST2747, complete genome, Sequence ID: CP007393.1Length: 4,998,910 Number
Escherichia coli 1303 plasmid p1303_95, complete sequence, Sequence ID: CP009168.1Length: 94959
- JANEWANGJK55
- in pBJ12_p1 shared **% nucleotide sequence identity with that of plasmid p0111_2 (GenBank accession no. AP010962.1), which was nearly identical to phage P1 in an enterohemorrhagic E. coli isolate from a patient in Japan. In addition, the replication protein RepA identified in the phage P1-like sequence was identical to that of bacteriophage P1 (GenBank accession no. NC_005856.1) from E. coli and other enteric bacteria.
excluding three tRNAs
- JANEWANGJK55
- Plasmid pHNFP460-1 (KJ020575) 99,868 bp in size,
Plasmid p42-2 (KT990220) 106,886 bp in size,12 IncF, composed of a typical IncFII-type backbone () and one distinct acquired DNA region spanning **
- JANEWANGJK55
- Phages are considered to play an important role in the horizontal transfer of DNA between microorganisms, as they can transfer resistant genes, mobile elements, and even plasmids. Recently, phage-like sequences were reported in plasmids from E. coli and Acinetobacter baumannii isoltes (10-12). Because these phage P1-like sequences were not present in pHNSHP45-2, they might incorporate into a plasmid of a E. coli ST189 isolate. The majority of the phage P1 genome was well conserved in pBJ12_p1. Genes related to replication, tail proteins, morphogenetic proteins, addiction systems, packing proteins, lytic proteins, regulatory proteins, and hypothetical proteins of the phage were detected in the pBJ12_p1 plasmid. However, most of the phage P1 sequences might be lysogenized, and its ** protein-coding genes were missing in pBJ12_p1. IntI1 and IS5075 occurred in pBJ12_p1 only and might play an important role in recombination between the plasmid and the phage.
Ref----Tn21; cre----IS5075.
BLAST the gaps….
- JANEWANGJK55
- . The “ISApl1-mcr-1-cassette” was found to be located in the E. coli S51 chromosome between the gene encoding for the outer membrane protein E and for a glutamate-5-kinase, respectively
