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- JANEWANGJK55
- 133 S. enterica serovar Indiana isolates were isolated
133 (**%) of 1320 serotyped Salmonella spp isolates in China were identified as S. Indiana.
133 S. enterica serovar Indiana isolates were isolated from 1320 samples in poultry slaughterhouses in 2012 in China.6
133 (**%) of 1320 serotyped Salmonella spp isolates in China were identified as S. Indiana.
- JANEWANGJK55
- The mcr-1 gene of the 133 MDR strains, were screened by PCR amplification, as described previously (Liu et al., 2016). The bacterial isolate was also assessed for the presence of the mcr-1 gene by PCR, as described previously2. Susceptibility testing was performed for S455 isolate by broth dilution (Oxoid, Thermo Fisher Scientific Inc.) according CLSI and EUCAST. Results were interpreted according to the criteria of CLSI.
- JANEWANGJK55
- pHNSHP45-2, pig feces= Class 1 integrons. In1185, In641.
pC629, chicken carcass= Class 1 integrons. In27 (dfrA12-orfF-aadA2), In54 (dfrA17-aadA5).
IS26-oqxAB-IS26; CTX-M genes; aac(6’)lb-cr-blaOXA-1-catB3-aar-3-sul1; floR; fosA3;
Further genetic screening of 63 oqxAB-positive Salmonella spp. isolates revealed that the majority carried IncHI2 plasmids, confirming that such plasmids play a pivotal role in dissemination of oqxAB in Salmonella spp.. The co-localization of blaCTX-M-9 group gene and oqxAB has been mainly described on IncFII plasmids harboured by E. coli isolates, in contrast to IncHI2 plasmids which have been previously reported on S. Indiana and S. enteritidis. Revealed that most of the oqxAB-positive Salmonella spp. were attributed to IncHI2 plasmids.
Plasmid pC629 was also recovered from one S. Indiana isolate C629, a carbapenem-resistant bacterium isolated from chicken carcass in China. The pC629 harboured *** MDR genes, including blaNDM-1, blaCTX-M, fosA and so on (Figure 1a).
- JANEWANGJK55
- pE15017 might be an additive example of IncI2-type plasmid with above two antibiotic resistance determinants.
Interestingly, four base pair (AACA, 1612-1615) is consistently missed in the ISEcp1-blaCTX-M-55-orf477 operon (3090 bp) in both pE15017 and pA31-12, in relative to the counterpart (3094 bp) in PHN122-1. In fact, the ISEcp1-blaCTX-M-55 transposition unit flanked by DR was also recently observed in the Salmonella plasmid pSCS23 (KU934209) as shown in Fig 3. (2016-Plos-gen-Feng)
- JANEWANGJK55
- pS455-4 exhibited nucleotide sequence homology to plasmids pColG from E. coli strain CA46 and K. pneumonia
- JANEWANGJK55
- Name Size Compare to
S455-Genome
S455-plasmid 1 222,458 bp pHNSHP45-2 (KU341381), pC629 (CP015725), pA3T (KX421096),
S455-plasmid 2 60,540 bp mcr-1 pHNSHP45 (64015 bp) (KP347127), pSCS23 (JAC) (KU934209)
S455-plasmid 3 38,906 bp aph(3’)-IIa pACN001-A (KC853434, 60,043 bp), pMR0516mcr (KX276657), pHNFP460-1 (KJ020575),
S455-plasmid 4 8,815 bp pColG (DQ286390)
