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- JANEWANGJK55
- http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0037152#pone.0037152-Zhang1
- JANEWANGJK55
- Materials and methods
Section of the bacterial isolate for study
The bacterial isolate was identified using the VITEK 2 compact platform (bioMérieux Vitek, Hazelwood, MO, USA) and 16S rDNA gene-based sequencing. The plasmid-borne carbapenemase- and ESBL-encoding genes were assessed by PCR.
- JANEWANGJK55
- Growth curve assays to assess plasmid-related fitness costs-
To assess any potential fitness costs associated with transmissible plasmids identified in the original isolates, growth curve profiles were determined for each transconjugant and compared with the plasmid-free recipient. Growth assays were initiated with 1:10 mixtures of independent overnight cultures of test isolate grown in fresh LB broth. Growth was measured at 37 °C for 24 h, using the Multiskan TM FC Microplate Photometer (Thermo Fisher Scientific). The OD610 nm was measured at 15-min intervals over a period of 24 h. The experiment was repeated three times.
- JANEWANGJK55
- Interestingly, the mcr-1 positive IncI2 plasmid was co-occurrence with the other plasmids with diverse replicon types such as IncHI2 and IncFII. 2016-Sci rep-fengyoujun-clonal. ISApl1 was present upstream of the mcr-1 gene, a ISApl1-mcr-1-orf-ISApl1 structure has been found in IncHI2-type plasmids and bacterial chromosomes with the ability to generate a circular intermediate harbouring ISApl1 and mcr-1 [8-11]2016-JAC-li.
