提出问题：Metabolic oscillations independent of the circadian clock, particularly at the subcellular level is unexplored. 

主要发现：Here, we reveal a metabolic rhythm of the essential coenzyme thiamine diphosphate (TDP) in the Arabidopsis nucleus. 

Fig. 1 Overview of thiamine metabolism in plants.

结果结论：We show there is temporal separation of the clock control of cellular biosynthesis and transport of TDP at the transcriptional level. Taking advantage of the sole reported riboswitch metabolite sensor in plants, we show that TDP oscillates in the nucleus. This oscillation is a function of a light-dark cycle and is independent of circadian clock control.

展望：The findings are important to understand plant fitness in terms of metabolite rhythms.

摘  要

在植物中，生长和发育的驱动力代谢稳态通过酶网络的动态行为来实现，其中很多酶的活性依赖于辅酶。生物钟能够影响植物中代谢物供应与需求之间的平衡。独立于生物钟的代谢节律，尤其是在亚细胞水平上的研究还很少。本文中，作者揭示了拟南芥细胞核中必不可少的辅酶硫胺素二磷酸TDP存在代谢节律。作者发现TDP的细胞生物合成与运输的生物钟控制在转录水平上存在时间隔离。利用植物中唯一报道的“核糖开关”代谢物传感器，作者发现TDP在细胞核中存在节律。该节律属于光/暗周期，并且独立于生物钟的调控。本文的研究结果有助于我们进一步理解植物在代谢物节律水平上的适应性。